Year: 2025 | Month: July-September | Volume: 10 | Issue: 3 | Pages: 144-150
DOI: https://doi.org/10.52403/gijhsr.20250315
Comparative Evaluation of Haematoxylin Stain Uptake in Prostate Glands of Cadmium Chloride-Exposed Wistar Rats
Blessing Emosho Ogeyemhe1, Akanbiola Iyadunni Oluwabunmi1, Ifueko Mercy Moses-Otutu1, Efosa Bolaji Odigie1
1Department of Medical Laboratory Science, School of Basic Medical Sciences, University of Benin, P.M.B. 1154, Benin City, Nigeria.
Corresponding Author: Efosa Bolaji Odigie
ABSTRACT
Haematoxylin stains are widely used in histopathology for visualizing tissue architecture, yet variations in preparation and uptake can influence diagnostic accuracy. This study compared the staining outcomes of different haematoxylin formulations on prostate glands of Wistar rats exposed to cadmium chloride across Nigeria’s six geopolitical zones. Thirty-five adult male rats were grouped and administered cadmium chloride (2 mg/kg, subcutaneously), while controls received distilled water. Prostate glands were harvested, weighed, and processed histologically using Harris and Cole’s haematoxylin stains sourced from the respective zones. Microscopic assessment showed that most groups retained normal histoarchitecture, including acini lined by ductal epithelium, eosinophilic secretions, papillary infoldings, and apical vacuoles. In the Southwest and South-south zones, staining was intensely dark with poor nuclear differentiation, whereas the North Central and Southeast zones demonstrated relatively clearer outcomes with occasional dehydration artifacts. The Northeast slides showed atrophic and poorly differentiated changes, while mild hyperplastic alterations appeared in the Northwest and Southeast zones. Overall, cadmium chloride exposure did not significantly disrupt prostate histoarchitecture, but distinct differences in haematoxylin stain uptake were observed across regions. Harris stains generally produced darker, less differentiated sections, while Cole’s stains yielded comparatively clearer images. These findings highlight the importance of stain quality and preparation in ensuring reliable histopathological interpretation and adherence to standards.
Keywords: Haematoxylin stain; Cadmium chloride; Prostate histology; Histopathology; Staining variability; Nigeria Geopolitical zones